Coding

Part:BBa_K1666000:Design

Designed by: Heming Wang   Group: iGEM15_NEFU_China   (2015-08-26)


LsrA of LuxS/AI-2 signaling pathway in Salmonalla


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 939
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lsrA gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).

Source

We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene LsrA(Gene ID: 1255601).

References

[1]Michiko E. Taga, Julia L. Semmelhack and Bonnie L. Bassler. The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in Salmonella typhimurium. Mol Microbiol, 2001, 42(3): 777–793

[2]Vendeville A, Winzer K, Heurlier K, et al. Making ‘sense’ of metabolism: autoinducer-2, LuxS and pathogenic bacteria. Nat Rev Microbiol, 2005, 3(5): 383-396

[3]Taga ME, Miller ST, Bassler BL. Lsr-mediated transport and processing of AI-2 in Salmonella typhimurium. Mol Microbiol, 2003, 50(4): 1411-1427.